為了解雞群中I群禽腺病毒(FAdV-I)的流行現(xiàn)狀,,從山東,、黑龍江,、吉林等地部分大規(guī)模養(yǎng)雞場(chǎng)采集病料,進(jìn)行病毒分離,、PCR鑒定及同源性分析,。結(jié)果顯示:從76份病料中分離到10株FAdV-I,分離率為13%,;10株FAdV-I中,,1株為血清4型,6株為血清8b型,,1株為血清9型,,2株為血清11型。結(jié)果表明,,山東,、黑龍江、吉林等地均存在一定的FAdV-I流行,,主要流行血清型已由4型轉(zhuǎn)變?yōu)?/span>8b型,,因此需要評(píng)估當(dāng)前的4型滅活苗能否有效防控這些地區(qū)的FAdV-I流行。
Isolation and Identificationand Homology Analysis on Group I Fowl Adenovirus
In order to recognize the prevalence ofgroup I fowl adenovirus(FAdV-I)in poultry,,samples were collected from several large-scalechicken farms in Shandong,,Heilongjiang and Jilin provinces for FAdV isolation,PCR assay and homology analysis. Resultsshowed that 10 FAdV-I strains were isolated from 76 samples with the positiverate reaching 13%. Among the 10 FAdV-I strains,,1 of them belonged to serotype 4,,6 of them belonged to serotype 8b,1 of them belonged to serotype 9,,and 2 of them belonged to serotype 11. Theresults indicated that certain FAdV-I infection existed in Shandong,,Heilongjiang and Jilin provinces,and the main serotypes had been transferredfrom serotype 4 to serotype 8b,,thus the current serotype 4 inactivated vaccines need to be evaluatedto determine if they could control the spread of FAdV-I effectively.
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